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. 2018 Jun 22;13(6):e0199666. doi: 10.1371/journal.pone.0199666

Fig 6. NK-4 down-regulates secretion of eotaxin by NHDF in response to IL-4 and/or TNF-α.

Fig 6

NHDF were stimulated with varying concentrations of IL-4 (A) or TNF-α (B) in the presence or absence (control) of 10 μM NK-4 for 48 h at 37°C. The effect of NK-4 on the secretion of eotaxin from NHDF stimulated with 10 ng/ml IL-4 and 5 ng/ml TNF-α was assessed (C and D). Concentrations of eotaxin in culture supernatants were measured by ELISA (A to C). Cell numbers of NHDF were determined by cell counting kit-8 (D). Results are the means ± S.D. of triplicate cultures. Results are representative of three independent experiments with similar results. *p < 0.05, **p < 0.01 compared with control cultures.