Figure 4.

Assessing the effect of HIF-1 inhibition on T-REx-HRE cell viability. (A) Cells were incubated in hypoxia for up to 72 h with or without 1 μg/mL dox. Cell viability was assessed with an MTT assay. (B) Cells were treated as in panel A with or without the addition of 3 mg/mL 2DG to the culture media. (C) Cells were treated as in panel B and incubated in hypoxia for 72 h. The ratio of cell viability to cytotoxicity was determined using a triplex reporter assay. (D) Cells were treated as in panel C and apoptotic cell death was assessed via a caspase 3/7 activity assay. Data are means (n = 3) ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.