Table 1. Oligonucleotide primer sequences used in the study .
Target Gene | Primer sequence (5'→3') | Amplicon size (bp) | Ref |
cagE |
F:5ʹ- TTGAAAACTTCAAGGATAGGATAGAGC-3ʹ R:5ʹ- GCCTAGCGTAATATCACCATTACCC-3ʹ |
500 | 21 |
cagA |
F: 5ʹ- AATACACCAACGCCTCCAAG-3ʹ R: 5ʹ- TTGTTGGCGCTTGCTCTC-3ʹ |
298 | 22 |
iceA1 |
F: 5ʹ- GTGTTTTTAACCAAAGTATC-3ʹ R: 5ʹ- CTATAGCCASTCTCTTTGCA-3ʹ |
247 | 23 |
iceA2 |
F: 5ʹ- GTTGTCGTTGTTTTAATGAA-3ʹ R: 5ʹ- GTCTTAAACCCCACGATTAAA-3ʹ |
120 | 24 |
VacA |
F: 5ʹ- GCCGATATGCAAATGAGCCGC-3ʹ R: 5ʹ- CAATCGTGTGGGTTCTGGAGC-3ʹ |
678 | 24 |
oipA |
F: 5ʹ- GTTTTTGATGCATGGGATTT-3ʹ R: 5ʹ- GTGCATCTCTTATGGCTTT -3ʹ |
401 | 25 |