Figure 5.

A kinase-dead Dbf2 localizes to SPBs. (B) Wild-type (K699), dbf2–2 (A852), dbf2–2 YcP DBF2-MYC (A3046), and dbf2–2 YcP Dbf2^{KI ->RT} -MYC (A3045) cells grown at 37°C. (A) dbf2–2 cells carrying a DBF2-MYC fusion or a DBF2-MYC fusion with the conserved K206 mutated to R (Dbf2^{KI ->RT}) were grown to exponential phase followed by shift to 37°C for 2 h and Dbf2 kinase activity was determined. (C) Localization of Dbf2^{KI ->RT} -Myc in exponentially growing cells (DBF2, YcP Dbf2^{KI ->RT} -MYC; A3044).