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. 2001 Oct;12(10):2961–2974. doi: 10.1091/mbc.12.10.2961

Figure 6.

Figure 6

Dbf2 localization and kinase activity in mutants defective for the mitotic exit pathway. Cells lacking a DBF2-MYC fusion (K699, no tag; lane 1), DBF2-MYC cells (A1931, wild-type, lane 2), tem1–3 DBF2-MYC (A 2135, lane 3), cdc5–1 DBF2-MYC (A 2107, lane 4), cdc15–2 DBF2-MYC (A 2096, lane 5), cdc14–3 DBF2-MYC (A 2179, lane 6) and cfi1:: URA3 DBF2-MYC (A 2156, lane 7) cells were arrested at the restrictive temperature (37°C) for 2 h. Dbf2-Myc proteins levels and Dbf2 kinase activity was determined (A). The graph in (A) shows the amount of Dbf2 kinase activity/Dbf2 protein with the numbers in the graph corresponding to lane numbers. Dbf2-Myc localization was analyzed in cdc15–2 DBF2-MYC (B) and cdc14–3 DBF2-MYC (C) mutants.