Table 1. Disordered regions and RNA-binding domains of proteins that drive the formation of membraneless organelles.
| Driver proteina | IDRsb | Enriched AAs | RNA binding | MLOs | References |
|---|---|---|---|---|---|
| DDX3_CAEEL (708) D0PV95 LAF-1/DDX3 |
1-202 620-708 |
9-168: R/G 624-691: G |
N-term IDR (RGG repeats) | P granule | [13, 29] |
| DDX4_HUMAN (724) Q9NQI0 DDX4 |
1-257 | 58-234: G | N-term IDR? | Nuage | [17] |
| FBRL_CAEEL (352) Q22053 FIB1/fibrillarin |
1-122 | 8-114: DMA/G | N-term IDR? Fibrillarin domain (119-346)? |
Nucleolus | [10] |
| FUS_HUMAN (526) P35637 FUS |
1-286 356-526 |
1-165: Q/G/S/Y 166-267: G 371-526: R/G |
RRM (287-365) | Stress granule Paraspeckle DNA damage site | [12, 14, 16, 47] |
| IF4F2_YEAST (914) P39936 eIF4G2 |
1-104 111-389 454-581 812-914 |
32-97: N 459-510: R/S 840-863: R/S |
MIF4G (567-810) | Stress granule | [14] |
| LSM4_YEAST (187) P40070 LSm4 |
88-187 | LSM (5-81) | P body | [14] | |
| NPM_XENLA (299) P07222 NPM1/nucleophosmin |
116-258 | 1-7: M 122-137: D/E 160-187: D/E |
NPM1-C (246-294) | Nucleolus | [10, 24] |
| PTBP1_HUMAN (531) P26599 PTB/HNRNP I |
316-323: A only | RRM (59-143) RRM (184-260) RRM (337-411) RRM (454-529) |
Nuclear speckle | [5] | |
| PUB1_YEAST (453) P32588 Pub1 |
1-64 211-330 410-453 |
8-16: Q 243-288: N 442-453: Q |
RRM (77-146) RRM (164-234) RRM (343-407) |
Stress granule | [14] |
| ROA1_HUMAN (372) P09651 HNRNPA1 |
177-372 | 195-372: G | RRM (16-85) RRM (107-176) C-term IDR |
Stress granule Paraspeckle | [14, 15] |
| TADBP_HUMAN (414) Q13148 TDP-43 |
258-414 | 274-413: G | RRM (106-172) RRM (193-243) |
Stress granule Paraspeckle | [15, 22] |
| TIA1_HUMAN (386) P31483 TIA-1 |
331-386 | RRM (9-77) RRM (108-178) RRM (216-280) |
Stress granule | [14, 60] | |
| WHI3_YEAST (661) P34761 WHI3 |
1-102 196-314 324-580 608-661 |
247-277: Q | RRM (540-613) | Stress granule | [19] |
| ROA2_HUMAN (341) P22626-2 hnRNPA2 |
171-341 | 190-341: G | RRM (11-80) RRM (102-171) |
Stress granule | [18, 23] |
| NPHN_HUMAN (1241) O60500 Nephrin |
1095-1171 | Nuclear body | [20] | ||
| Q9TXM1_CAEEL (862) Q9TXM1 MEG-3 |
1-382 395-606 |
S | Entire length | P granule | [21] |
| G5EBV6_CAEEL (693) G5EBV6 PGL-3 |
518-693 | RGG repeats (634-693) | P granule | [32] |
a
Each protein is identified by the UniProt entry name (with length given in parentheses) and accession number, as well as the common name.
b
Disorder was predicted by PONDR-VSL2 [77], with the threshold score set at 0.8 for reliability.