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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Stem Cell Rev. 2018 Aug;14(4):525–534. doi: 10.1007/s12015-018-9805-1

Figure 2. Mean fluorescence intensity, transfection efficiency, and time kinetics of eGFP expression.

Figure 2

A) Flow cytometric histograms show eGFP expression pattern and gating threshold used for subsequent analyses of eGFP. Shaded histogram represents non-transfection control.

B) Percentage of eGFP-positive cells at 24 hours after transfection. Mean±SD from 3 experiments is shown.

C) Time kinetic study of eGFP levels. eGFP mean fluorescence intensity (MFI) from all samples were plotted as 0 at time 0. Green and pink * indicate significant different in eGFP expression compared to their single 3′UTR mimics. δ indicates significance compared to all others. Results are mean ±SD from 3 experiments

D) Representative flow cytometric dot plot showing eGFP expression in WA01 cell line at 1 week after transfection with 5′eGFP3′. E) Non-linear curve fit was performed using the first-order kinetic model (A(t)=A0e−kt). eGFP MFI were plotted on a log scale vs. time. Data represents mean±SD from 3 experiments.