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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Stem Cell Rev. 2018 Aug;14(4):525–534. doi: 10.1007/s12015-018-9805-1

Figure 3. Generation of Endothelial cells with ETV2 modRNA.

Figure 3

A) Representative flow cytometric dot plots and immunofluorescence images showing endothelial markers and AcLDL uptake on ETV2-transfected PSCs at 24 hours after transfection. Cells were transferred to collagen-coated plate and cultured in endothelial medium for live staining. AcLDL = acetylated-low density lipoprotein. Scale bar = 50 μm.

B) Percentage of CD144+ cells shown in the gate from A). Results are mean±SD from 3 experiments.

C) Yield of total live CD144+ cells from ETV2 transfected cultures. Mean±SD from 3 experiments is shown.

D) Expansion of endothelial cells generated in ETV2-transduced cultures. Representative picture shows endothelial cells at passage 3 after transfection. Scatter dot plot showing accumulative number of endothelial cells after expansion. Scale bar = 100 μm.