Fig. 1.

Zika vaccine design. a A phylogenetic tree for ZIKV genomes up to November 2015; blue and red labels represent the African and Asian lineages of ZIKV, respectively. b Conservation homology of an Asian consensus sequence versus all genomic sequences depicted in Supplementary Fig. 1. The circle represents the ZIKV-BR strain used for the challenge experiment. c Schematic representation of the Zika immunogen versions used in this study; red box represents the tPA leading sequence. d Restriction enzyme analysis of the plasmid DNA vaccine constructs that were further cloned into ChAdOx1 vector; a 3.3 kb band size represents the DNA vaccine plasmid back bone. e Expression of ZIKV immunogens by western immunoblotting using an anti-ZIKV envelope antibody. M molecular marker, C control, E empty plasmid. Uncropped images are shown in Supplementary Fig. 2