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. 2018 Jun 22;8:9539. doi: 10.1038/s41598-018-27687-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mycobacterial complementation vector pFLAG for constitutive low-level expression. (A) Schematic overview of the pFLAG vector depicting relevant features. (B) Detection of Rv1410-3xFLAG produced from integrative vector pFLAG_attP in the presence or absence of apramycin. Total cell lysate supernatants were separated as a two-fold dilution series by SDS-PAGE and Rv1410-3xFLAG was detected by Western blotting using an anti-FLAG antibody. The uncropped Western blot image is shown in Supplementary Fig. S1.