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. 2018 Jun 22;9:2434. doi: 10.1038/s41467-018-04919-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Microfluidic setup. a Chip design. A total of 16 syringes with aqueous samples are connected to the inlets in the microfluidic chip via tubing (10 with compounds, 2 with medium to generate single drug and control conditions, 2 for barcoding, 1 for the cell suspension, 1 with Caspase-3 substrate to detect apoptosis). Other 2 inlets in the microfluidics chip are used for carrier oil (FC-40) and mineral oil. The braille display unit is used to control the valves (red coloured circles) and regulate the flow coming from the aqueous phase syringes, resulting in different combinations. Plugs are collected in a tube connected to the outlet. b Experimental setup. Microfluidic chip mounted onto a Braille display aligning the microfluidic channels with the braille valves. c, d Cross section of a valve mounted on the Braille display in the open and closed configuration. e Combinatorial plugs production. Single compounds and pairwise combinations are automatically generated. First barcode (BC) plugs are generated followed by the corresponding assay plugs for each condition. f Storage of plugs. Plugs are stored in PTFE tubing for incubation and readout purposes. g Array of binary barcodes in a microfluidic channel. Plugs contain two different dyes (bright colour indicating a “1”, dark color indicating a “0”) were used to generate binary numbers 40–50