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. 2018 Jun 22;8:9513. doi: 10.1038/s41598-018-27753-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cysteine is the prevalent thiol in ascitic fluid from patients with ovarian cancer. Thiols quantification in ascitic fluid from patients with ovarian cancer. (A) Total thiols concentration. (B) Free total thiols concentration. (C) Thiols concentration bound to proteins. (D) Percentage of thiols concentration bound to proteins. Cys – cysteine, HCys- Homocysteine, CysGly – Cysteinylglycine, GluCys – Glutamylcystein, GSH – Glutathione, CysSSP – S-cysteinylated proteins, HcySSP – S-homocysteinylated proteins, CysGlySSP – S-cysteinylglycinylated proteins, GluCysSSPS – Glutamylcysteinylated proteins, GSSP – S-glutathionylated proteins, RSSP – total S-thiolated proteins. Thiol concentration was normalized by protein concentration. Results are shown as median. The *represent the statistical significance in relation to Cysteine concentration. *p < 0.05, **p < 0.01, ***p < 0.001 (independent samples Kruskal Wallis One-way ANOVA with multiple comparisons).