Fig. 2.

Separation of Ycf3-HA preparation on SDG ultracentrifugation. The purified Ycf3-HA was concentrated and subjected to SDG ultracentrifugation, and the resulting gradient was fractionated and was analyzed by immunoblot using anti-PsaA, Y3IP1, Ycf4, Ycf3, and PSAF antibodies. Ycf3 and Y3IP1 were fractionated in the upper region of the gradient, whereas PsaA and Ycf4 were detected at positions around 700 kDa. Separation of chlorophyll–protein complexes from the WT thylakoid extracts was shown on the top as a reference to show apparent molecular sizes on the gradient. LHCII-T: trimeric LHCII, LHCI-M: monomeric LHCII