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. 2018 Jun 22;8:9554. doi: 10.1038/s41598-018-27900-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot of anti-FLAG immunoprecipitation. Htt was immunoprecipitated from (a) S. cerevisiae and (b) S. pombe Htt-expression strains using an anti-FLAG mAb coupled to magnetic beads, as described in Methods. Equivalent quantities (25 OD) of whole cell lysate, input, unbound, and IP-bound samples were loaded and detected with anti-FLAG mAb. Note that the two sides of panel A are cropped from different regions of the same gel. The complete gel is shown at the end of the Supplementary Information document.