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. 2018 Jun 22;8:9497. doi: 10.1038/s41598-018-27918-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Migration of hPTECs was impaired by DMOG. (A) hPTECs were seeded in Ibidi barriers in 8-well slides and grown to confluence. After removal of the barriers, cells were allowed to migrate into the open space for 7 h. Cells were stained for E-cadherin (red) and F-actin (green). Scale bar: 30 µm. (B) Distal hPTECs were seeded as described above. Pictures of the wound were taken directly after removal of the barrier and 15 h later. (C) Distal hPTECs were seeded as described above. Cells were treated with DMOG (1 mM) 24 h prior to removal of the barriers. Movement of the cells was monitored using the program provided by the manufacturer (IncuCyte^R, Essen Biosciences). Based on the confluency data, migration speed was calculated in arbitrary units per h, covering an early (2–5 h) and late (7–15 h) time-frame. In each experiment, migration of control cells between 2 and 5 h was set to 1. The graph summarizes data (means ± SD) of 3 experiments. *p < 0.05 compared to control cells.