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Basolateral sorting requires a di-leucine motif. Expression of point mutants NPP1/AL (a–c), NPP1/LA (d–f), and NPP1/AA (g–i) in MDCK cells. Each pictures is a sum of three 0.5-μm confocal microscopy xy sections from the bottom (a, d, and g), middle (b, e, and h), and top (c, f, and i) of the cells. Bars, 20 μm.
