Table 2.

Composition and LightCycler settings of the BSI-PCR assay

Channel settings (Ex-Em)	Probe label	Multiplex 1	Multiplex 2	Multiplex 3	Multiplex 4
440–488 nm	Atto425	Pan-Aspergillus	E. coli	S. aureus	Staphylococcus genus
465–510 nm	FAM	Gram-positive	E. faecium	E. faecalis	Enterococcus genus
498–580 nm	HEX/Yakima Yellow	Gram-negative	A. baumannii	P. aeruginosa	Klebsiella species
533–610 nm	CalFluor610	3Candida a	–	C. krusei	C. albicans
533–640 nm	CalFluor635	C. glabrata	mecA	S. pneumoniae	CTX-M1,9
618–660 nm	Atto647N	IAC	IAC	IAC	IAC

All PCR reactions were performed on a LightCycler 480 II (Roche Diagnostics).

IAC internal amplification control, Ex excitation wavelength setting, Em emission wavelength setting

^a3Candida PCR detects C. albicans, C. tropicalis C. parapsilosis