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. 2010 Jun 20;13(6):612–616. [Article in Chinese] doi: 10.3779/j.issn.1009-3419.2010.06.009

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人脑组织(阳性对照)TRPC和IPO8荧光定量PCR扩增产物的琼脂糖凝胶电泳结果。采用所设计的引物(表 1)分别检测到TRPC1-7和IPO8在人脑组织中的表达。电泳显示各基因扩增产物条带单一、大小与预期分子量一致,表明引物特异性好

Agarose gel electrophoresis of TRPC and IPO8 qPCR products. Human brain cDNA was used as the positive control to validate the specificity of TRPC1-7 and IPO8 qPCR primers. Agarose gel electrophoresis indicated a single band with expected size of each qPCR product (TRPC1-7 and IPO8)