Skip to main content
. 2001 Oct;12(10):3016–3030. doi: 10.1091/mbc.12.10.3016

Figure 4.

Figure 4

Predicted amino acid sequence of DdAIF and comparison with hAIF. (a) Amino acid sequence of DdAIF. Boxes indicate the MLS, NLSs, and DBM. (b) DdAIF immunodetection: 1) 293T cells transfected with control vector; 2) 293T cells overexpressing DdAIF (AIF); 3) Dictyostelium cell extract; 4) Dictyostelium cell extract immunodepleted with a nonimmune serum; 5, Dictyostelium cell extract immunodepleted with anti-DdAIF antibody; 6) Dictyostelium cell extract immunodepleted with anti-DdAIF antibody in the presence of DdAIF-specific peptides (20 μM peptides). (c) CLUSTAL-W was used to generate an alignment of DdAIF with hAIF. DdAIF is ∼33% identical and ∼60% similar to hAIF.