Table 1.
Primers for Target Genes
| Gene | Primer | Sequences |
|---|---|---|
| SCN5A | Sense | 5′‐TGGTTGTCATCCTCTCCATCGT‐3′ |
| Antisense | 5′‐ATGAGGGCAAAGAGCAGCGT‐3′ | |
| SCN5A | ChIP sense | 5′‐TCTGAGTGAGAAACAGTGGGTG‐3′ |
| ChIP antisense | 5′‐CCACCCTCTTGAGCCACATAC‐3′ | |
| SCN5A | P2kb‐S‐Mlu I | 5′‐ATAACGCGTAGGAGAGTGAAAACCCCACTTCTG‐3′ |
| P2kb‐AS‐Bgl II | 5′‐ATAAGATCTACTCGCTCACCTGCTGGTCCC‐3′ | |
| HuR | Sense | 5′‐GACCATGACCCAGAAGGACG‐3′ |
| Antisense | 5′‐CGGATAAACGCAACCCCTCT‐3′ | |
| MEF2C | Sense | 5′‐TACAACGAGCCGCATGAGAG‐3′ |
| Antisense | 5′‐GGAATCGTCCGCATCGGG‐3′ | |
| MEF2C | RIP sense | 5′‐CATGTTGCAGGTTCAACGTTATTT‐3′ |
| RIP antisense | 5′‐CCATTACCGGGTCTGTCCA‐3′ | |
| 28S RNA | Sense | 5′‐GTGACGCGCATGAATGGA‐3′ |
| Antisense | 5′‐CCCTTGGCTGTGGTTTCG‐3′ | |
| ACTB | Sense | 5′‐AAGAGCTACGAGCTGCCTGAC‐3′ |
| Antisense | 5′‐ACAGGACTCCATGCCCAGGAA‐3′ | |
| Luciferase | Sense | 5′‐ACACCCGAGGGGGATGATAA‐3′ |
| Antisense | 5′‐TCTCACACACAGTTCGCCTC‐3′ | |
| EGFP | Sense | 5′‐GGACGACGGCAACTACAAGA‐3′ |
| Antisense | 5′‐TTGTACTCCAGCTTGTGCCC‐3′ |
ACTB indicates beta‐actin; ChIP, chromatin immunoprecipitation; EGFP, enhanced green fluorescent protein; HuR, Hu antigen R; MEF2C, myocyte enhancer factor‐2C; RIP, ribonucleoprotein immunoprecipitation.