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In vivo interactions of methyl CpG binding domain (MBD) proteins with the luc‐CYP11B2 promoter with or without DNA methylation (chromatin immunoprecipitation assay). A, Schema of the luc‐CYP11B2 promoter constructs with or without DNA methylation. B, Recruitment of MBD proteins. R1 and R2 denote regions 1 and 2 amplified to measure MBDs interaction with CpG1 (luc‐Ad1) and CpG2 (luc‐Ad5), respectively. Values are means±SD. *P<0.05.
