Table 2.
NoShift assay | |
Anti‐CREB1 | sc‐58; Santa Cruz Biotechnology, Santa Cruz, CA |
Anti‐NR4A1 (NGFI‐B) | CBX00289; Cosmo Biolabs Inc, Tokyo, Japan |
Anti‐NR4A2 (NURR1) | sc‐991; Santa Cruz Biotechnology |
Anti‐NR4A3 (NOR1) | PP‐H7833‐00; Perseus Proteomics Inc, Tokyo, Japan |
Anti‐NR5A1 (SF1) | A generous gift of Dr Ken‐ichiro Morohashi |
Anti‐MECP2 | pAb‐052‐050; Diagenode sa, Liège, Belgium |
Chromatin immunoprecipitation quantitative PCR | |
Anti‐MECP2 | pAb‐052‐050; Diagenode sa |
Anti‐MBD1 | ab2846; Abcam, Cambridge, UK |
Anti‐MBD2 | A‐1007; Epigentek Group Inc, Brooklyn, NY |
Anti‐CREB1 | sc‐240; Santa Cruz Biotechnology |
Anti‐phospho‐CREB1 (Ser133) | #17‐10131; Millipore, Billerica, MA |
Anti‐NR4A1 (NGFI‐B) | ab13851; Abcam |
CREB1 indicates cAMP response element binding protein1; MBD, methyl‐CpG‐binding domain protein; MECP, methyl‐CpG binding protein; NGFI‐B, nerve growth factor‐induced clone B; NOR1, neuron‐derived orphan receptor 1; NURR1, NUR‐related factor 1; SF1, steroidogenic factor 1.