Figure 2.

Subnuclear localization of DDX1 with respect to other nuclear bodies. HeLa cells were immunolabeled with anti-DDX1 antibody (2923) and either anti-SC-35 (A), anti-PML (B), anti-Sm (C), anti-Sam68 (D), or anti-CstF-64 (E) antibodies. Adjacent localization of a DDX1 focus and PML body is indicated by the arrowhead in B, merged image. Adjacent localization of DDX1 foci and Cajal bodies are indicated by the arrowheads in C, merged image. All 3 DDX1 foci colocalize with cleavage bodies in E, merged image. The images were collected with the use of a Zeiss LSM 510 confocal laser scanning microscope. Bar, 5 μm.