Figure 3.

Coimmunoprecipitation of DDX1 and CstF-64. Nuclear extracts from HeLa cells and RB522A cells (1 mg) were precleared and incubated with 3 μl of either preimmune serum or anti-DDX1 antibody (2910). Complexes were precipitated with protein A-agarose beads and run on an 8% SDS-polyacrylamide gel. Proteins were transferred to nitrocellulose and detected with anti-DDX1 antibody (top), anti-CstF-64 antibody (middle), and anti-Sam68 antibody (bottom). For comparison, we have loaded 2.5% of the supernatant (S) from each immunoprecipitation reaction next to the immunoprecipitation (IP) lanes. IgG molecules are indicated by the asterisks. Size markers (in kDa) are indicated on the right.