Figure 6.

Disappearance of DDX1 and CstF-64 foci during mitosis. HeLa cells were immunolabeled with anti-DDX1 antibody (2923) and anti-CstF-64 antibody. The cells were mounted with DAPI to visualize the chromatin morphology. (A) Both DDX1 and CstF-64 foci are present during early prophase (indicated by the arrowheads). (B) Only DDX1 foci are present by late prophase (arrowheads). (C) Neither DDX1 nor CstF-64 foci can be detected during metaphase. (D) DDX1 foci reappear during G1 (arrowheads); however, CstF-64 foci are not observed. Although the overall DDX1 signal remains constant throughout mitosis, there is a decrease in CstF-64 signal. For example, compare the metaphase cell with adjacent cells in C. Bar, 5 μm.