Fig. 4.

Effects of zoledronic acid on mRNA and protein expression of M2 macrophage markers in IL-4-treated THP-1 cells. THP-1 cells were treated overnight with 100 ng/ml PMA, washed with PBS, and incubated with or without Zol for 24 h. THP-1 cells were then treated with 50 ng/ml IL-4. mRNA expression was detected by the real-time RT-PCR. Protein expression was detected by western blotting. Open bars represent IL-4-treated THP-1 cells; filled bars represent Zol and IL-4-treated THP-1 cells. a Zol did not affect the mRNA and protein expression of CD206 in IL-4-treated THP-1 cells. b Zol did not affect the mRNA expression of IL-10 and IL-1ra in IL-4-treated THP-1 cells