Fig. 2.

A rarefaction curve (a), box plot (b) and PCoA (c) for each primer pair. For analysis, respective sequences of each primer pair were clustered at 97% similarity using QIIME. For rarefaction plots, sequences were rarefied with 10,000 sequences per sample and the Chao1 index was plotted. PCoA was generated using unweighted unifrac metrics. Box plots were generated using BoxplotR