Table 1.
Top 10 list of non-Glomeromycota OTUs with the highest betweenness within the metacommunity networks
| OTU | Score | Phylum | Class | Order | Family | Genus | Category | NCBI Blast top hit | Accession | Cover | Identity |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Full (8 sites) | |||||||||||
| F_0042* | 1.000 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella humilis | KP714537 | 100% | 100% |
| F_0381 | 0.844 | Basidiomycota | Tremellomycetes | Trichosporonales | Trichosporonaceae | Cryptococcus | Others_unknown | Saitozyma podzolica† | KY320605 | 92% | 99% |
| F_0079 | 0.679 | Ascomycota | Sordariomycetes | Hypocreales | Nectriaceae | – | Saprotroph/endophyte | Ilyonectria protearum | NR_152890 | 99% | 100% |
| F_0489 | 0.576 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella sp. | KM113754 | 100% | 100% |
| F_0010 | 0.411 | Ascomycota | Leotiomycetes | – | Myxotrichaceae | Oidiodendron | Ericoid_mycorrhizal | Oidiodendron maius | LC206669 | 100% | 100% |
| F_0368 | 0.403 | Basidiomycota | Malasseziomycetes | Malasseziales | Malasseziaceae | Malassezia | Others_unknown | Malassezia restricta | KT809059 | 100% | 100% |
| F_0623 | 0.382 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella gamsii | KY305027 | 100% | 100% |
| F_1188 | 0.334 | Basidiomycota | Tremellomycetes | Trichosporonales | Trichosporonaceae | Cryptococcus | Others_unknown | Saitozyma podzolica† | KY320605 | 92% | 99% |
| F_0007 | 0.333 | Ascomycota | Sordariomycetes | Diaporthales | Melanconidaceae | Melanconiella | Saprotroph/endophyte | Melanconiella elegans | KJ173701 | 100% | 85% |
| F_0485 | 0.288 | Ascomycota | Sordariomycetes | Hypocreales | Hypocreaceae | Trichoderma | Saprotroph/endophyte | Trichoderma sp. | HG008760 | 100% | 100% |
| Northern 4 sites (cool-temperate) | |||||||||||
| F_0042* | 1.000 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella humilis | KP714537 | 100% | 100% |
| F_0034* | 0.795 | Ascomycota | Eurotiomycetes | Chaetothyriales | Herpotrichiellaceae | Cladophialophora | Saprotroph/endophyte | Cladophialophora chaetospira | KF359558 | 100% | 99% |
| F_0079* | 0.767 | Ascomycota | Sordariomycetes | Hypocreales | Nectriaceae | – | Saprotroph/endophyte | Ilyonectria protearum | NR_152890 | 99% | 100% |
| F_0015* | 0.763 | Ascomycota | – | – | – | – | Others_unknown | Cadophora orchidicola | KX611558 | 100% | 99% |
| F_0202* | 0.749 | Ascomycota | Eurotiomycetes | Chaetothyriales | Herpotrichiellaceae | Cladophialophora | Saprotroph/endophyte | Cladophialophora chaetospira | HQ871875 | 100% | 99% |
| F_0195* | 0.611 | Ascomycota | Eurotiomycetes | Chaetothyriales | Herpotrichiellaceae | Cladophialophora | Saprotroph/endophyte | Cladophialophora chaetospira | EU035405 | 100% | 100% |
| F_0181* | 0.580 | Ascomycota | Leotiomycetes | Helotiales | Dermateaceae | Pezicula | Endophyte | Pezicula melanigena | LC206665 | 100% | 99% |
| F_0010 | 0.545 | Ascomycota | Leotiomycetes | – | Myxotrichaceae | Oidiodendron | Ericoid_mycorrhizal | Oidiodendron maius | LC206669 | 100% | 100% |
| F_0103* | 0.538 | Ascomycota | Eurotiomycetes | Chaetothyriales | Herpotrichiellaceae | Cladophialophora | Saprotroph/endophyte | Cladophialophora chaetospira | EU035403 | 100% | 97% |
| F_0489* | 0.534 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella sp. | KM113754 | 100% | 100% |
| Southern 4 sites (warm-temperate and subtropical) | |||||||||||
| F_0381* | 1.000 | Basidiomycota | Tremellomycetes | Trichosporonales | Trichosporonaceae | Cryptococcus | Others_unknown | Saitozyma podzolica† | KY320605 | 92% | 99% |
| F_0042* | 0.569 | – | – | Mortierellales | Mortierellaceae | Mortierella | Saprotroph/endophyte | Mortierella humilis | KP714537 | 100% | 100% |
| F_0610* | 0.460 | Ascomycota | Sordariomycetes | Hypocreales | Hypocreaceae | Trichoderma | Saprotroph/endophyte | Trichoderma spirale | KU948158 | 100% | 100% |
| F_1188* | 0.443 | Basidiomycota | Tremellomycetes | Trichosporonales | Trichosporonaceae | Cryptococcus | Others_unknown | Saitozyma podzolica† | KY320605 | 92% | 99% |
| F_0029 | 0.414 | Ascomycota | Eurotiomycetes | Chaetothyriales | Herpotrichiellaceae | – | Others_unknown | Cladophialophora sp. | LC189029 | 100% | 99% |
| F_0017 | 0.319 | Ascomycota | – | – | – | – | Others_unknown | Scleropezicula sp. | KT809119 | 100% | 98% |
| F_0007 | 0.314 | Ascomycota | Sordariomycetes | Diaporthales | Melanconidaceae | Melanconiella | Saprotroph/endophyte | Melanconiella elegans | KJ173701 | 100% | 85% |
| F_0485 | 0.303 | Ascomycota | Sordariomycetes | Hypocreales | Hypocreaceae | Trichoderma | Saprotroph/endophyte | Trichoderma sp. | HG008760 | 100% | 100% |
| F_0112 | 0.266 | Basidiomycota | Agaricomycetes | Thelephorales | Thelephoraceae | Tomentella | Ectomycorrhizal | Tomentella stuposa | KR019860 | 100% | 98% |
| F_0073 | 0.258 | Ascomycota | Sordariomycetes | – | – | – | Others_unknown | Rhexodenticula acaciae | KY173442 | 94% | 95% |
In each of the three metacommunity-scale networks examined (full, cool-temperate, and warm-temperate/subtropical), fungal OTUs were ranked based on their betweenness centrality scores. As taxonomic information of Glomeromycota OTUs with high betweenness scores was redundant (e.g., Glomus spp. or Glomeraceae spp.), the top 10 list of non-Glomeromycota OTUs is shown. Taxonomy information of each OTU was inferred based on the query-centric auto-k-nearest-neighbor algorithm of reference database search [59, 60] and subsequent taxonomic assignment with the lowest common ancestor algorithm [66]. The results of the NCBI nucleotide Blast are also shown. For simplicity, the functional groups of fungi inferred with the program FUNGuild [67] were organized into several categories. See Additional file 4; Data S4 for details of the categories and for full results including Glomeromycota and other fungal OTUs
*Fungal OTUs classified as metacommunity hubs (mean local betweenness > 0.5; metacommunity betweenness > 0.5)
†Synonym, Cryptcoccus podzolica