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. Author manuscript; available in PMC: 2018 Jun 23.
Published in final edited form as: J Med Chem. 2017 Jul 13;60(14):6364–6383. doi: 10.1021/acs.jmedchem.7b00561

Figure 2.

Figure 2

Titration curves for anti-HCV activity (EC50, triangle in blue) and cytotoxicity (CC50, circle in red) of selected leads. Cell-culture adapted HCV (HCVcc) harboring a luciferase reporter gene (HCV-Luc, genotype 2a) was used to infect Huh7.5.1 cells in the presence of increasing concentrations of test compound. Viral infection and replication were measured by luciferase signal 48 h after treatment. Cytotoxicity was evaluated in parallel with the ATP-based cell viability assay (ATPlite). The results are mean from three replicates ± SEM. The EC50 and CC50 values were calculated with GraphPad Prism 5.0 software using nonlinear regression. Curves and values for each compound were from single representative experiments.