FIGURE 3.

Effect of ONO-8713 on OGD-induced cell death in the CA1 region of cultured hippocampal slices. Organotypic mouse hippocampal slice cultures were placed in an air-tight chamber that was flushed with anoxic gas (5% CO₂, 95% N₂) for 1 h at 37°C. Then the cultures were transferred to normal culture medium with or without 1 μM ONO-8713 and incubated for 24 h. The PI fluorescence was obtained at 24 h after OGD, and the maximal PI fluorescence was obtained by stimulating the cultures for 24 h with a lethal amount of NMDA (100 μM). (A) Fluorescence images (4X magnification) showing PI staining in hippocampal slices subjected to OGD for 1 h. Images were taken before OGD (Base), 24 h after OGD (OGD), and 24 h after NMDA incubation (MAX). (B) Histogram representing CA1 neuronal damage measured by PI fluorescence intensity. The neuronal damage was significantly lower in ONO-8713-treated slices (n=27) than in vehicle-treated slices (n=24) after OGD-induced injury. ***P <0.001 as compared with the vehicle-treated group.