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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Nat Biomed Eng. 2018 Jan 22;2(2):95–103. doi: 10.1038/s41551-017-0187-5

Figure 3. CARG is highly specific in targeting S. aureus infections.

Figure 3

(a–b) Skin infection in mice was generated by subcutaneous inoculation of S. aureus bacterial suspension. The sham-infected control mice received subcutaneous injection of PBS. At 72 h post-infection, FAM-CARG was intravenously injected via the tail vein and allowed to circulate for 30 min to home to the skin abscesses. (a) Immunofluorescence microscopy of skin sections from mice with (top) or without (bottom) S. aureus infection at low (20X) magnification. High (40X) magnification of CARG homing to infected skin section (Dashed square site). FAM-CARG (green), S. aureus (red) and cell nuclei stained with DAPI (blue). Representative images of at least five skin sections from each group (n = 3 mice per group) are shown. Scale bar: 50 μm (low magnification); 20 μm (high magnification). (b) Imunohistochemical staining for the FAM label reveals intense signal (brown) from FAM-CARG in S. aureus-infected skin as compared to healthy skin. Sections were counterstained with DAPI (blue). Scale bar: 200 μm.