Table 1.

Sequences of oligonucleotide primers used for PRRs signaling pathway genes mRNA levels analysis.

Gene producta		Primer	Product size (bp)
	Directionb	Sequence (5′–3′)
GADPH	F	CCAGAACATCATCCC TGCTT	229
	R	GTCCTCAGTGTAGCCCAGGA
TLR2	F	TCACTTGTCTAACTTATCATC CTCTTG	162
	R	TCAGCGAAGGTGTCATTATTGC
TLR4	F	GCCATCGCTGCTAACA TCATC	108
	R	CTCATACTCAAAGATACAC CATCGG
TLR9	F	GTGGAACTGTTTTGGCATC	199
	R	CACAGCACTCTGAGCTTTGT
NOD1	F	ACCGATCCAGTGAGC AGATA	140
	R	AAGTCCACCAGCTCCATGAT
NOD2	F	GAGCGCATCCTCTTAACTTTCG	66
	R	ACGCTCGTGATCCGTGAAC

^aGADPH, glyceraldehyde-3-phosphate dehydrogenase; TLR, toll-like receptor; NOD, nucleotide-binding oligomerization domain. ^bF, forward; R, reverse.