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. 2018 Jun 18;9:1345. doi: 10.3389/fimmu.2018.01345

Figure 1.

Figure 1

Culture medium impacts patient-derived glioblastoma (GBM) cells’ morphology and phenotype. Cell morphology of (A) P3, (B) 2012-018, (C) BG5, and (D) BG7 GBM cells cultured in NB (a) magnification 100×, scale bar 50 µm, (b) magnification 200×, scale bar 50 µm; and in Dulbecco’s modified eagle medium (DMEM) (c) magnification 100×, scale bar 50 µm, (d) magnification 200×, scale bar 50 µm. Histograms showing intracellular expression of nestin on (E) P3, (F) 2012-018, (G) BG5, and (H) BG7 GBM cells cultured in NB (upper histograms) and in DMEM (lower histograms). Dark gray histograms—fluorescent minus one control and light gray histogram—nestin.