Table 2.
Polymerase chain reaction (PCR) primers used in this study.
| Primer name | Sequence (5′—3′) | Target gene | Length (bp) | Reference |
|---|---|---|---|---|
| 27F | 5′-AGAGTTTGATCCTGGCTCAG-3′ | 16S rRNA | 1400–1500 | Li et al., 2007 |
| 1492R | 5′-ACGGTTACCTTGTTACGACTT-3′ | |||
| K1F | 5′-TSAAGTCSAACATCGGBCA-3′ | PKS-I | 1200–1400 | Ayuso-Sacido and Genilloud, 2005 |
| M6R | 5′-CGCAGGTTSCSGTACCAGTA-3′ | |||
| KSα | 5′-TSGCSTGCTTGGAYGCSATC-3′ | PKS-II | 600 | Metsa-Ketela et al., 1999 |
| KSβ | 5′-TGGAANCCGCCGAABCCTCT-3′ | |||
| A3F | 5′-GCSTACSYSATSTACACSTCSGG-3′ | NRPS | 700–800 | Ayuso-Sacido and Genilloud, 2005 |
| A7R | 5′-SASGTCVCCSGTSCGGTAS-3′ | |||