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. 2018 Jun 18;8:218. doi: 10.3389/fonc.2018.00218

Figure 2.

Figure 2

Targeting intracellular reactive oxygen species (ROS) promotes leukemic cell differentiation. (A,B) Measurement of ROS production by chemiluminescence following fMLF stimulation in the presence or absence of histamine dihydrochloride. (A) A representative curve showing the respiratory burst by dimethyl sulfoxide (DMSO)-differentiated wild-type (WT) or NOX2-KO PLB-985. (B) ROS production by undifferentiated and DMSO-differentiated WT or NOX2-KO PLB-985 cells. (C–E) Fluorescent measurement of intracellular ROS in WT and NOX2-KO PLB-985 using flow cytometry: (C) cytoplasmic ROS, and (D,E) mitochondrial ROS. Antimycin A was used to trigger mitochondrial ROS production. (F) WT and NOX2-KO PLB-985 cells were cultured in the presence or absence of luminol or isoluminol for 5 days and the percentage of CD11b+ cells was determined by flow cytometry. Two-tailed paired or unpaired t-tests, one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.