Table 2.
Antibodies used for western blot.
| Immunoreagent | Dilution | Manufacturer |
|---|---|---|
| Primary antibody | ||
| Mouse-anti-neurofilament L (clone DA2) |
1:1000 | Thermo Fisher, Waltham, MA, United States |
| Rabbit-anti-neurofilament L | 1:2000 | Synaptic Systems, Göttingen, Germany |
| Rabbit-anti-α-internexin | 1:5000 | Abcam, Cambridge, United Kingdom |
| Mouse-anti-β-actin | 1:2000 | Cell Signaling, Danvers, MA, United States |
| Secondary antibody | ||
| HRP-horse-anti-mouse IgG | 1:10000 | Vector Laboratories, Burlingame, CA, United States |
| HRP-goat-anti-rabbit IgG | 1:10000 | Vector Laboratories |