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. 2018 Jun 18;9:754. doi: 10.3389/fphys.2018.00754

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Copyright © 2018 Xue, Jia, Wang, Yang, Wang, Gao and Hao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Quiescent PSCs stain positively for desmin by IF. (Hoechst 33258 staining, and Alexa Fluor 488 staining for desmin, original magnification, ×200). (B) Activated PSCs stain positively for α-SMA by IF (Hoechst 33258 staining, and Alexa Fluor 594 staining for α-SMA, original magnification, ×200). (C) Quiescent PSCs have an angular appearance, contained lipid droplets by oil red O staining. (Original magnification, ×400). (D) Senescence is shown in PSCs with cytoplasmic blue staining of SA-β-gal. (Immunocytochemistry; original magnification, ×400) (Xue et al., 2017b).