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| Mouse | Primers (5′ to 3′) | Product size, bp |
|---|---|---|
| Tph1fl/fl | GGATCCTAACCGAGTGTTCC | Wild type: 350 |
| GCACACCACCAACTCTTTCC | Floxed: 450 | |
| Adiponectin-Cre | TGCCATGTGAGTCTGCCTTT | Cre+: 700 |
| AACCAGCGTTTTCGTTCTGC | ||
| Htr3a KO | TGGATGTGGAATGTGTGCGAG | Wild type: 400 |
| AACAGCTATGCAGAAATGAAGTT | Knockout: 210 | |
| GGCTGACTGCGTAGAATAAAGG |
Tphfl/fl, tryptophan hydroxylase 1 floxed; Htr3a, 5-hydroxytryptamine receptor 3A; KO, knockout.
