Table 2. Sequences of primers used in quantitative real-time polymerase chain reaction.
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| Acaca | CAGTAACCTGGTGAAGCTGGA | GCCAGACATGCTGGATCTCAT |
| Acly | CCCTCTTCAGCCGACATACC | CTGCTTGTGATCCCCAGTGA |
| Actb | GGTACCACCATGTACCCAGG | GAAAGGGTGTAAAACGCAGC |
| Agpat1 | GCGCAATGTCGAGAACATGA | TCATTCCAAGCAGGTCGAGG |
| Apob | TACTTCCACCCACAGTCCCCT | CCTTAGAAGCCTTGGGCACAT |
| Cd36 | TGGCCAAGCTATTGCGACAT | ACACAGCGTAGATAGACCTGC |
| Cpt1a | AGCTCGCACATTACAAGGACA | CCAGCACAAAGTTGCAGGAC |
| Dgat1 | GGATCTGAGGTGCCATCGTC | ATCAGCATCACCACACACCA |
| Dgat2 | CATCATCGTGGTGGGAGGTG | TGGGAACCAGATCAGCTCCAT |
| Fasn | AAGCGGTCTGGAAAGCTGAA | AGGCTGGGTTGATACCTCCA |
| Gpam | CCACAGAGCTGGGAAAGGTT | GTGCCTTGTGTGCGTTTCAT |
| Lpin1 | CATACAAAGGCAGCCACACG | CGGGGTTCAGTCCCTTGTAG |
| Me1 | GACCCGCATCTCAACAAGGA | CAGGAGATACCTGTCGAAGTCA |
| Mlxipl | AAGTTGCTATGCCGGGACAA | ATGACAGCCTCAGGTTTCCG |
| Mogat1 | TTGACCCATGGTGCCAGTTT | GTGGCAAGGCTACTCCCATT |
| Mttp | TGCTTCCGTTAAAGGTCACACA | CTTGCGGTTTTCCTTTGCCC |
| Nr1h3 | GCAGGACCAGCTCCAAGTAG | CCCTTCTCAGTCTGCTCCAC |
| Pparg | GGTGTGATCTTAACTGCCGGA | GCCCAAACCTGATGGCATTG |
| Ppargc1a | GCCCAGGTACGACAGCTATG | ACGGCGCTCTTCAATTGCTT |
| Scd1 | AGAGTCAGGAGGGCAGGTTT | GAACTGGAGATCTCTTGGAGCA |
| Srebp1c | GGAGCCATGGATTGCACATT | GGCCCGGGAAGTCACTGT |
Acaca, acetyl-CoA carboxylase alpha; Acly, ATP citrate lyase; Actb, actin beta; Agpat1, 1-acylglycerol-3-phosphate O-acyltransferase 1; Apob, apolipoprotein B; Cpt1a, carnitine palmitoyltransferase 1a; Dgat1, diacylglycerol O-acyltransferase 1; Dgat2, diacylglycerol O-acyltransferase 2; Fasn, fatty acid synthase; Gpam, glycerol-3-phosphate acyltransferase; Lpin1, lipin 1; Me1, malic enzyme 1; Mlxipl, MLX interacting protein-like (ChREBP, carbohydrate response element binding protein); Mogat1, monoacylglycerol O-acyltransferase 1; Mttp, microsomal triglyceride transfer protein; Nr1h3, nuclear receptor subfamily 1, group H, member 3 (LXR, liver X receptor); Pparg, peroxisome proliferator activated receptor gamma; Ppargc1a, Pparg coactivator 1 alpha; Scd1, stearoyl-CoA desaturase 1; Srebp1c, sterol regulatory element binding transcription factor 1c.