FIG. 3.

The number of transient receptor potential melastatin 4 (Trpm4)+ cells in the hippocampus peaks at 6 h post-injury and reduces throughout the first 8 weeks following injury. (A) Representative confocal micrograph of the rodent hippocampus 6 h following central fluid percussion injury (CFPI). The indicated region demarcates the hippocampal fissure from the gray matter of the hippocampus. Bar graphs depicting the total number of Trpm4+ cells/100 um² of the white matter of the hippocampal fissure, the non-fissure gray matter of the hippocampus, or the entire hippocampus at (B) 6 h (sham n = 4; 6 h traumatic brain injury [TBI] n = 5), (C) 1 day (sham n = 2; 1 day TBI n = 4), (D) 1 week (sham n = 2; 1 week TBI n = 6), (E) 4 weeks (sham n = 3; 4 weeks TBI n = 5) and (F) 8 weeks (sham n = 3; 8 weeks TBI n = 4) post-TBI. The trend toward more Trpm4+ cells at 1 day post-injury is nonsignificant; however, the increase in Trpm4+ cells at both 1 week and 4 weeks post-injury is significant. The number of Trpm4+ cells reduces to levels indistinguishable from sham by 8 weeks post-CFPI. Sham represented by black bars and CFPI represented by gray bars. T-test against sham for each region of interest at each time-point; error bars represent standard error of the mean. *p < 0.05.