FIG. 5.

Transient receptor potential melastatin 4 (Trpm4) expression is not correlated to cell damage/death following diffuse moderate traumatic brain injury (TBI). (A) Representative light micrographs of the hippocampus from sham-injured or TBI animals at 6 h, 1 day, 1 week, 4 weeks, and 8 weeks. Inset depicts damaged cells demonstrating pyknotic nuclei and eosinophilic cytoplasm from a positive control (arrows). (B) Bar graph depicting the average number of damaged or dying cells/100 um² in the entire hippocampus. Note that at no time-point do the numbers of damaged/dying cells increase above sham levels. (C) Scatterplot illustrating the lack of correlation between average number of Trpm4+ cells and average number of damaged/dying cells in the entire hippocampus. Each point represents an individual animal sustaining either sham injury or central fluid percussion injury (CFPI). Sham n = 7, 6 h n = 5, 1 day n = 5, 1 week n = 4, 4 weeks n = 4, 8 weeks n = 4. Sham represented by black bars and CFPI represented by gray bars. Analysis of variance with Bonferroni post hoc test; error bars represent standard error of the mean scale bar (A) 200 μm; inset 50 μm.