FIG. 7.

Astrocytes expressing transient receptor potential melastatin 4 (Trpm4) have a greater somatic cytoplasmic area indicating cellular swelling. (A) Bar graph depicting the average area of the somatic cytoplasm surrounding the nucleus. Trpm4 expression nearly doubled the astrocyte cytoplasmic area, compared with Trpm4- astrocytes. (B) Representative electron micrograph of astrocytes from the hippocampal gray matter 4 weeks post–central fluid percussion injury labeled against Trpm4 (white arrows; red pseudo color). The arrows indicate immunoreactivity against Trpm4. The astrocyte in the middle of the electron micrograph (black arrow head; blue pseudo color) is a Trpm4- astrocyte located between two Trpm4+ astrocytes. N indicates the nucleus of each cell. Traumatic brain injury (TBI), three animals Trpm4- n = 57 cells, Trpm4+ n = 110 cells. Analysis of variance; error bars represent standard error of the mean. *p < 0.05. Scale bar 5 μm.