FIG. 7.

(A) Representative photomicrographs illustrating anti-Von Willebrand Factor (vWF) staining in the ipsilateral cortex in males and females. vWF was present in vessels as confirmed with t-lectin co-labeling (see insets). There was more vWF staining in the vessels of the 1 day post-injury (dpi) males compared with Sham and 7 dpi males, and compared with the 1 dpi females (scale bar: 1 mm, inset: 50 μm). (B) β-catenin staining in the ipsilateral cortex in males and females was localized to vessels, confirmed by t-lectin co-labeling (see insets). There was more β-catenin staining in the vessels of 1 dpi males compared with Sham and 7 dpi males, and compared with the 1 dpi females (scale bar: 1 mm, inset: 50 μm). (C) Quantification of vWF staining in ipsilateral cortex revealed increased staining in males at 1dpi compared with Sham and 7 dpi (*p < 0.05) but not in females between groups. There was significantly higher vWF staining in the males compared with the females at 1 dpi (**p < 0.01). (D) Quantification of β-catenin staining in ipsilateral cortex exhibited the same profile as vWF. There was increased staining in males at 1 dpi compared with Sham and 7 dpi (**p < 0.01). In females, there was a trend toward a decrease between Sham and 1 dpi mice (p = 0.06). There was significantly higher β-catenin staining in the males compared with the females at 1 dpi (****p < 0.0001).