Table 2.
Slopes of qRT-PCR curves for reference genes.
| Gene | Equation of standard curves | Slope | Regression coefficient | Amplification efficiency |
|---|---|---|---|---|
| GAPDH | y = -3.131x + 31.876 | -3.131 | R2 = 0.999 | 108.654% |
| 18S | y = -3.226x + 23.333 | -3.226 | R2 = 0.993 | 104.142% |
| ACT | y = -3.277x + 21.576 | -3.277 | R2 = 0.996 | 101.927% |
| UBI | y = -3.302x + 23.212 | -3.302 | R2 = 0.990 | 100.86% |
| GTP | y = -3.262x + 29.435 | -3.262 | R2 = 0.999 | 102.559% |
| H2A | y = -3.185x + 23.602 | -3.185 | R2 = 0.996 | 106.054% |
| TUA | y = -3.287x + 17.442 | -3.287 | R2 =0.997 | 101.483% |
| UBC | y = -3.198x + 21.166 | -3.198 | R2 =0.996 | 105.454% |
| EF1 | y = -3.244x + 17.020 | -3.244 | R2 =0.998 | 103.367% |
| TUB | y = -3.274x + 28.293 | -3.274 | R2 =1 | 102.048% |