TABLE 1 .
Percentages of CreA::GFP, Fbx23::GFP, CkiA::GFP, and GskA::GFP nuclear localizationa
| Background | % nuclear localization |
|
|---|---|---|
| Glucose | Xylan | |
| CreA::GFP | ||
| A. nidulans wild type | 95.61 | 14.02 |
| A. nidulans Δfbx23 | 92.31 | 94.93 |
| A. nidulans Δfbx47 | 63.79 | 52.58 |
| GFP | ||
| A. nidulans Fbx23::GFP | 0.00 | 0.00 |
| A. nidulans CkiA::GFP | 100.00 | 81.58 |
| A. nidulans GskA::GFP | 8.00 | 45.25 |
a
Strains were grown for 16 h at 22°C in minimal medium supplemented with either glucose or xylan before GFP cellular localization was assessed. Nuclei were counted for at least 100 hyphal germlings under each condition and subjected to Hoechst 33258 staining in order to confirm GFP colocalization.