FIG 2 .
Cwp19 is a lytic transglycosylase. (A) RP-HPLC separation of soluble PG fragments released from M. lysodeikticus PG after incubation with buffer (Untreated) or His6-Cwp19 (Cwp19-treated). Annotated peaks were collected and their masses determined by MALDI-TOF MS. (B) MALDI-TOF/TOF tandem MS spectrum of the reaction product in peak C. The [M+Na]+ parent ion (m/z 957.55) was selected for fragmentation, and the inferred structure is represented at the bottom of the panel. The fragments were also detected as sodiated ions.