TABLE 2 .
Enrichment of four S25DHs starting from 5 g (wet weight) recombinant T. aromatica
| Enzyme and fraction | Protein (mg) |
Sp act (nmol min−1 mg−1)a |
Enrichment factor |
Yield (%) |
|---|---|---|---|---|
| S25DH1 | ||||
| Soluble fraction | 645 | 11.2 | 100 | |
| DEAE + Reactive Red | 6.6 | 395 | 35.3 | 36 |
| S25DH2 | ||||
| Soluble fraction | 546 | 9.3 | 100 | |
| DEAE + Reactive Red | 9 | 151 | 16.2 | 27 |
| S25DH3 | ||||
| Soluble fraction | 590 | 0.21 | 100 | |
| DEAE + Reactive Red | 10.2 | 4.6 | 21.9 | 38 |
| S25DH4 | ||||
| Soluble fraction | 552 | 6.4 | 100 | |
| DEAE + Reactive Red | 10 | 97 | 15.1 | 27 |
a
Specific activities were determined by the conversion of cholest-4-en-3-one for S25DH1, S25DH2, and S25DH4 and of campest-4-en-3-one for S25DH3.