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. 2018 Jun 20;11:3573–3581. doi: 10.2147/OTT.S158868

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© 2018 Shen et al. This work is published and licensed by Dove Medical Press Limited

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Changes in library complexity.

Notes: Dashed lines indicate mean FPKM of the samples with different RIN values. (A) RIN=9.0 and RIN=5.3; (B) RIN=9.0 and RIN=6.3; (C) RIN=9.0 and RIN=7.3; (D) density plots of FPKM values among all 4 individuals with RIN values of 9.0, 7.3, 6.3 and 5.3, respectively. “R” represents the samples that were used to extract RNA immediately, and then the RNAs were stored at room temperature (25°C) for different times (30 min–24 h).

Abbreviations: FPKM, fragments per kilo base transcript per million; RIN, RNA integrity number.