Table S1.
General sequencing information
| Sample ID | RIN values | Raw reads | Clean reads | Mapped reads |
|---|---|---|---|---|
| 1R | 9.0 | 140091314 | 138029858 (98.5%) | 98691349 (71.5%) |
| 1R0 | 7.3 | 36736206 | 35168258 (95.7%) | 16318072 (46.4%) |
| 1R1-1 | 6.3 | 70823028 | 67866162 (95.8%) | 20248585 (29.8%) |
| 1R1-2 | 6.3 | 46824070 | 45188720 (96.5%) | 25456871 (56.3%) |
| 1R2-1 | 5.3 | 81378518 | 79891672 (98.2%) | 40536056 (50.7%) |
| 1R2-2 | 5.3 | 47012320 | 44489560 (94.6%) | 18049383 (40.6%) |
| 2Q1-1 | 7.0 | 52268928 | 33542190 (64.2%) | 6020397 (17.9%) |
| 2Q2-1 | 6.8 | 51389032 | 48389792 (94.1%) | 13299809 (27.5%) |
| 2Q3-2 | 6.3 | 49909838 | 44839786(89.8%) | 9244780 (20.6%) |
| 2Q4-1 | 5.5 | 55786686 | 47479870 (85.1%) | 17967837 (37.8%) |
| 2Q4-2 | 5.5 | 32527560 | 27984948 (86.0%) | 9635122 (34.4%) |
| 2Q5-1 | 5.5 | 67066026 | 56807680 (84.7%) | 16362545 (28.8%) |
| 2Q5-2 | 5.5 | 39266224 | 33130436 (84.4%) | 10601740 (32.0%) |
Notes:
1
The samples were used to extract RNA immediately after collection and stored at room temperature for different times to obtain the RNAs whose RIN values could be matched to the samples stored at 4°C, and then were chosen for the subsequent steps.
2
The samples were stored at 4°C for 4 hours, 1 day, 3 days, 5 days and 7 days prior to RNA extraction.
Abbreviation: RIN, RNA integrity number.