Figure 2.

The effects of miR-145 overexpression on the progression of LSCC.

Notes: TU212 and AMC-HN-8 cells were transfected with miR-145 or miR-con for gain-of-function analyses. (A) Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of miR-145 expression in treated TU212 and AMC-HN-8 cells. (B) MTT assay was conducted to detect cell proliferation at 24 h, 48 h and 72 h in treated TU212 and AMC-HN-8 cells. (C) Flow cytometry analysis was performed to analyze apoptosis of treated TU212 and AMC-HN-8 cells. Transwell migration (D) and invasion (E) assays were applied to determine the migration and invasion capabilities of treated TU212 and AMC-HN-8 cells; magnification: 200×. *. < 0.05.